Screening and analysis of host factors interacting with NS1 proteins of influenza A virus based on the proteome chip

doi:10.19803/j.1672-8629.20230463

Abstract

Abstract: Objective The human proteins interacting with influenza A virus (IAV) NS1 protein were obtained and analyzed to confirm the affected host biological processes and the core human proteins in the course of infection, providing thoughts for the development of drugs against influenza A virus. Methods NS1 sample and Biotin sample were hybridized with HuProt™ human proteome chip respectively, and the host protein bound to NS1 protein was screened on condition that both of repeated trials are matching Z-Score ≥ 3. The significantly specific proteins were further selected using a strict criteria (IMean_Ratio ≥ 1.4). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analysis were performed to evaluate these targets and MCODE was performed to explore the core proteins. Results A total of 195 significant specific proteins were obtained. Analysis results showed that these proteins were mainly involved in mRNA processing, RNA binding and protein binding, RNA degradation and amino acid biosynthesis. Among the four core proteins DDX6, HSPD1, PKLR and MTHFD1, DDX6 is related to RNA synthesis and translation. NS1 protein can promote viral infection by regulating IAV and host RNA, suggesting that DDX6 may be indispensable in this process. Although the other three proteins not clearly linked to influenza viruses, they can play a crucial role in the infection of other RNA viruses. Conclusion The human proteins bound to NS1 are mainly enriched in the process of RNA synthesis, processing, transcription, etc. The core proteins have potential to become new targets for anti-IAV, which need to be further verified by subsequent experiments.

Key words: influenza A virus, NS1 protein, proteome chip, bioinformatics, host factors, pathway

CLC Number:

R978
R994.11

ZHANG Yu, ZHANG Wei, GAO Shuangrong, CHEN Mengping, WANG Yaxin, XU Yingli, CAO Shan, ZHAO Ronghua, BAO Lei, LI Shuran, SUN Jing, BAO Yanyan, GENG Zihan, GUO Shanshan, JI Zuen, CUI Xiaolan. Screening and analysis of host factors interacting with NS1 proteins of influenza A virus based on the proteome chip[J]. Chinese Journal of Pharmacovigilance, 2024, 21(1): 40-44.

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