The Mechanism of Psoralea Fructus Induced Idiosyncratic Liver Injury Based on Metabolomics

doi:10.19803/j.1672-8629.2021.11.04

Abstract

Abstract: Objectiv eTo study the mechanism of Psoralea Fructus induced idiosyncratic injury based on metabolomics, and to screen related biomarkers and metabolic pathways. Methods A rat model of idiosyncratic liver injury was induced by caudal vein injection of LPS (LPS). Rats were randomly divided into the control group (CMC group), Psoralea Fructus group (BGZ group), lipopolysaccharide group and BGZ+LPS group. The serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities were analyzed. Global metabolomics profiling of the serum was detected by UPLC-QTOF/MS. The characteristic biomarkers and corresponding metabolic pathways were analyzed by Progenesis QI (v2.4), SIMCA v13.0, MetaboAnalyst v 5.0 and other software. Results Compared with the control group, there were no significant changes in ALT or AST in the BGZ group, but there was an increase in ALT and AST in the BGZ+LPS group. Furthermore, serum metabolite spectra in the CMC, BGZ, LPS and BGZ+LPS groups were significantly separated from OPLS-DA. Fourteen potential metabolomics biomarkers were screened that were associated with Psoralea Fructus induced idiosyncratic injury, involving seventeen pathways. Conclusion Psoralea Fructus induced liver injury is idiosyncratic liver injury, and its mechanism is possibly related to the regulation of sphingolipid metabolism, tyrosine metabolism and other metabolic pathways.

Key words: Psoralea Fructus, idiosyncratic liver injury, metabolomics, biomarkers, metabolic pathway

CLC Number:

R917.1

WANG Xiaoyan, LI Weixia, ZHANG Hui, ZHANG Mingliang, WU Yali, CAO Zhanxia, NI Wenjuan, CHEN Yulong, LI Kun, FENG Keran, TANG Jinfa. The Mechanism of Psoralea Fructus Induced Idiosyncratic Liver Injury Based on Metabolomics[J]. Chinese Journal of Pharmacovigilance, 2021, 18(11): 1014-1019.

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