Abstract: Abstract Objective To evaluate the consistency of chemiluminescence microparticle immunoassay (CMIA) and fluorescence polarization immunoassay assay (FPIA) in determination of antiepileptic plasma drugs concentration with valproic acid as an example. Methods CMIA and FPIA methods were applied to determine the plasma concentration of valproic acid in the same sample, respectively. Then the consistency of the two methods with Passing-Bablokregression and Bland-Altman method was investigated. Results The regression equation of the two determination results by CMIA (y) and FPIA (x) was y =1.0049x-0.9851(R2=0.978,n=318), which showed the correlation of the two methods was good. Bland-Altman analysis showed that the consistency of the two methods for determining was good. Conclusion CMIA and FPIA methods for determination of valproate plasma concentrations had good correlation, but there was systematic error. In the clinical treatment, we should pay attention to make some adjustment and choice.

Key words: CMIA, FPIA, anti-epileptic drugs, plasma concentration, consistency