Feasibility study on grape seed proanthocyanidins as a new ophthalmic antibacterial agent

doi:10.19803/j.1672-8629.20220398

Abstract

Abstract: Objective To investigate the bacteriostatic ability of grape seed proanthocyanidins in eye drops and provide experimental basis for the development of new antibacterial agents for eye drops. Methods The antibacterial ability and antibacterial efficacy of the original antibacterial agents and grape seed proanthocyanidins in eye drops were determined with reference to the general rule 1121 of the Ⅳ volume of the Chinese Pharmacopoeia, 2020 edition, and the ocular irritation of healthy rabbits was evaluated with reference to the compilation of guiding principles for preclinical research of new drugs (western medicines) issued by the national health and Family Planning Commission. In addition, in order to study the advantages of grape seed oligoprocyanidins as bacteriostatic agents, the cytotoxicity of grape seed procyanidins on HCEC cells and the inhibition of influenza A virus H1N1 were investigated by MTT colorimetry. Results With grape seed oligomeric proanthocyanidins as antibacterial agent, the reasonable addition was 0.10 mg·mL^-1, and the antibacterial ability could reach grade 0.10 mg·mL^-1 grape seed procyanidins aqueous solution had no irritation to rabbit eyes and had a certain inhibitory effect on influenza A (H1N1) virus. Conclusion Grape seed proanthocyanidin can be used as one of the antibacterial agents in eye drops.

Key words: grape seed proanthocyanidins, bacteriostatic efficacy, eye drops, bacteriostatic agents, feasibility

CLC Number:

R961

YIN Xiang, WANG Xi, SHI Chunhui, WANG Zongwei, WANG Lin, YAO Shangchen, XU Mingzhe. Feasibility study on grape seed proanthocyanidins as a new ophthalmic antibacterial agent[J]. Chinese Journal of Pharmacovigilance, 2022, 19(11): 1174-1180.

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