Single dose toxicity of Ficus microcarpa in mice

doi:10.19803/j.1672-8629.2022.03.07

Abstract

Abstract: Objective To study the changes of indexes of pathology, hematology and biochemistry under single-dose toxicity in mice treated with a median lethal dose (LD₅₀) or maximum tolerance dose (MTD) of Ficus microcarpa so as to evaluate the safety of Ficus microcarpa. Methods In LD₅₀ tests, the mice were randomly divided into 6 groups and given by gavage different doses of Ficus microcarpa. The general condition of mice and the morphological changes of viscera were observed after 7 days. In MTD experiments, the mice were randomly divided into 3 groups and given three times by gavage different drugs at different doses. The changes in toxic reactions and in hematological and biochemical indexes were observed and recorded within 14 days. Results There was no death of mice in LD₅₀ tests, suggesting that LD₅₀ could not be calculated. The MTD was calculated as 70.4 g·kg^-1 by the crude dose. In addition, histopathological observations revealed that the organs of mice in each group did not exhibit pathological reactions. And compared with the blank control group and the equivalent dose group, there was no statistical difference in hematological or biochemical index in the maximum tolerance dose group (P>0.05), and no obvious acute toxic reactions or death caused by Ficus microcarpa were observed. Conclusion Ficus microcarpa is quite safe.

Key words: Ficus microcarpa, single-does toxicity, maximum tolerance dose (MTD), histopathology

CLC Number:

R994.1

GAO Zhanwang, ZHANG Xin, MO Zunhui, WANG Lingli, YI Zhibiao. Single dose toxicity of Ficus microcarpa in mice[J]. Chinese Journal of Pharmacovigilance, 2022, 19(3): 265-269.

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