中国药物警戒 ›› 2010, Vol. 7 ›› Issue (10): 613-616.

• 综述 • 上一篇    下一篇

现代分析技术在中药毒理学中的地位和作用

孙蓉1,郑丽娜2,朱兰兰2, 杨倩3   

  1. 1.山东省中医药研究院,山东济南250014；
    2.天津中医药大学,天津300193；
    3. 山东中医药大学,山东济南250355
  • 收稿日期:2010-04-19 修回日期:2016-03-12 出版日期:2010-10-08 发布日期:2016-03-09
  • 作者简介:孙蓉,女,研究员,硕士生导师,中药药理与毒理研究。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）；国家自然科学基金项目（30672649）；山东省科技平台建设项目（2008GG2NS02021）；山东省科技公关关键技术研究课题（2007GG2NS02073）；山东省国际合作引智项目（L20083700336）

The Status and Role of Modern Analytical Techniques in the Toxicology of Traditional Chinese Medicine

SUN Rong1 ,ZHENG Li-na2 ,ZHU Lan-lan2, YANG Qian3   

  1. 1.Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2.Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2010-04-19 Revised:2016-03-12 Online:2010-10-08 Published:2016-03-09

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摘要： 目的 探讨现代分析技术在中药毒理学研究中的地位和作用。方法 对国内近十几年来相关文献进行整理、分析与归纳。结果 紫外分光光度法（UV）、原子吸收光谱法（AAS）、荧光光谱法、高效液相色谱法（HPLC）、气相色谱法（GC）、高效液相色谱-质谱联用技术（HPLC-MS）、气相色谱- 质谱联用技术（GC-MS）和分子生物学技术是目前中药毒理学研究中常用的分析技术,其中HPLC、GC-MS、HPLC-MS 应用最广泛。结论 现代分析技术在中药体内过程研究中发挥着重要作用,主要用于血液或肝、肾等重要组织中有毒物质的浓度测定；而且随着现代分析技术的发展和分析仪器的灵敏度的提高,必将促进中药毒理学的进一步发展。随着现代毒理学中新技术、新理论的建立与发现,现代分析技术必将在中药毒效动力学（TD）和毒代动力学（TK）研究中占据不可或缺的地位,发挥着不可替代的作用。

关键词: 现代分析技术, 中药毒理学

Abstract: Objective To approach the status and roles of modern analytical techniques in researching for toxicology of Traditional Chinese Medicine. Methods Collect, and summarize domestic related literature of the last decade. Results Ultraviolet Spectrophotometry(UV), Atomic Absorption Spectrometry(AAS), Fluorescence Spectroscopy(FS), High Performance Liquid Chromatography(HPLC), Gas Chromatography(GC), High Performance Liquid Chromatogr-aphy-Mass Spectrometry(HPLC-MS), Gas Chromatography -Mass Spectrometry(GC-MS) and Molecular Biology Techniques ( MBT) are commonly used in toxicology of Traditional Chinese Medicine sdudies, in which HPLC, GC -MS, HPLC -MS are the most widely used. Conclusion Modern analytical techniques in the study of vivo process of Traditional Chin-ese Medicine plays an important role, mainly for the determination of the concentration of toxic substances in blood or liver, kidney and other important tissue; And with the developmentof modern analytical techniques and the improvement of the sensitivity of analytical instruments, they will promote the further development of toxicology of Traditional Chinese Medicine. With the establishment and discoveries of new theories and new technology of modern toxicology, modern analytical techniques will play an more important role in Toxicodynamics (TD) and Toxicokinetics(TK) studies of Traditional Chinese Medicine, and plays an irreplaceable effect.

Key words: modern analytical techniques, toxicology of Traditional Chinese Medicine

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