中国药物警戒 ›› 2010, Vol. 7 ›› Issue (7): 385-389.

• 基础研究 •    下一篇

山豆根不同组分对小鼠急性肝毒性“量-时-毒”关系研究

杨倩1, 郑丽娜2, 谢元璋1, 孙蓉3*   

  1. 1山东中医药大学,山东 济南 250355;
    2天津中医药大学,天津 300193;
    3山东省中医药研究院,山东 济南 250014
  • 收稿日期:2016-02-22 修回日期:2016-02-22 出版日期:2010-07-08 发布日期:2016-02-22
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理研究。E-mail：sunrong107@163.com
  • 作者简介:杨倩,女,在读硕士研究生,中药药理与毒理研究。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目（2009CB522802）; 山东省科技平台建设项目课题（2008GG2NS02021）

Study on The “Dosage-Time-Toxicity” Relationship of Hepatotoxicity Induced by Different Components of Radix et Rhizoma Sophorae Tonkinensis in Mice

YANG Qian1, ZHENG Li-na2, XIE Yuan-zhang1, SUN Rong3,*   

  1. 1Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    2Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China
  • Received:2016-02-22 Revised:2016-02-22 Online:2010-07-08 Published:2016-02-22

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摘要： 目的 研究山豆根水提组分及醇提组分单次给药致小鼠急性肝毒性的“量-时-毒”关系。方法 “时–毒”关系研究：小鼠灌胃一定剂量的山豆根水提、醇提组分,分别于给药后不同时间检测血清丙氨酸氨基转移酶（ALT）、门冬氨酸氨基转移酶（AST）水平及肝、脾、胸腺等脏器指数的变化,光学显微镜下进行肝脏病理学检查。“量–毒”关系研究：给小鼠灌胃不同剂量的山豆根水提、醇提组分,分别于药后6h、4h按“时-毒”研究方法对小鼠进行相应处理。结果 小鼠血清ALT、AST水平分别在灌胃较高剂量的山豆根水提、醇提组分后6h、4h达到高峰,给药后12h肝脏指数均升至最高,给药后48h均可恢复至正常值;山豆根水提组分剂量在（1.789~3.494）g·kg-1之间、醇提组分在（2.779~5.427）g·kg-1之间,肝脏可产生明显病理损伤,且随着剂量增大ALT、AST水平升高显著。光学显微镜下观察小鼠肝细胞出现不同程度的核固缩、变性等病理改变。结论 小鼠单次灌胃一定剂量的山豆根水提组分或醇提组分可造成急性肝毒性损伤,并呈现明显的“量-时-毒”关系。

关键词: 山豆根, 肝毒性, “, 量-时-毒”, 关系

Abstract: Objective Study the "dosage-time-toxicity" relationship of hepatotoxicity induced by different components of Radix et Rhizoma Sophorae Tonkinensis(RRST) in mice. Methods Study on the "time-toxicity"relationship: Ig water extract and alcohol extract of RRST of certain dosage to mice and the changes of the level of ALT, AST in serum and organ index of liver,spleen and thoracic gland were tested at different time points after administration, the same time the hepar-pathology test was performed with light microscope. Study on the "dosage-toxicity"relationship: Ig water extract and alcohol extract of RRST of different dosages to mice and the corresponding treatment was performed at 6h/4h after administration in accordance with the method of study on"time-toxicity"relationship. Results The study on the "time-toxicity" relationship indicated that the level of ALT, AST in serum reached to the peak at 6h/4h after once administration of water extract and alcohol extract of RRST of higher dosage and the liver index rised to its topmost both at 12h.They all recovered to normal value at 48h after ig. The study on the "dosage-toxicity" relationship indicated that conspicuous hepartotoxical injury can be induced by water extract of RRST of (1.789~3.494)g·kg-1 and alcohol extract of (2.779~5.427)g·kg-1 and the level of ALT, AST increased significantly with the increase of dosage. Karyopycnosis and degeneration of different degrees of hepatic cells can be seen with the light microscope. Conclusion Once oral administration of water extract and alcohol extract of RRST with large dosage may induce acute hepatotoxical injury in mice and show an obvious "dosage-time-toxicity" relationship.

Key words: Radix et Rhizoma Sophorae Tonkinensis, hepatotoxicity, "dosage-time-toxicity" relationship

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