中国药物警戒 ›› 2024, Vol. 21 ›› Issue (4): 404-407.
DOI: 10.19803/j.1672-8629.20230283

• 基础与临床研究 • 上一篇    下一篇

佛芍颗粒对新斯的明致大鼠痉挛性腹痛的作用研究

李舒冉1, 郭姗姗1, 包蕾1, 赵荣华1, 孙静1, 耿子涵1, 鲍岩岩1, 张敬升1, 徐英利1, 吕耀中2,3, 王振中2,3, 崔晓兰1,*, 王红梅2,3#   

  1. 1中国中医科学院中药研究所生物安全实验室,北京 100700;
    2中药制药过程控制与智能制造技术全国重点实验室,江苏 连云港 222000;
    3 江苏康缘药业股份有限公司,江苏 连云港 222000
  • 收稿日期:2023-05-08 出版日期:2024-04-15 发布日期:2024-04-18
  • 通讯作者: *崔晓兰,女,博士,研究员·博导,中药防治感染性疾病机制研究。E-mail: xlcui@icmm.ac.cn,#为共同通信作者。
  • 作者简介:李舒冉,女,博士,助理研究员,中药抗感染与神经药理研究。
  • 基金资助:
    国家自然科学基金资助项目（82141206）; 中央级公益性科研院所基本科研业务费专项资金资助（ZZ15-YQ-035; ZXKT21031）; 中国中医科学院科技创新项目（CI2021B015）

Effect of Foshao granules on spastic abdominal pain in rats

LI Shuran1, GUO Shanshan1, BAO Lei1, ZHAO Ronghua1, SUN Jing1, GENG Zihan1, BAO Yanyan1, ZHANG Jingsheng1, XU Yingli1, LYU Yaozhong2,3, WANG Zhenzhong2,3, CUI Xiaolan1,*, WANG Hongmei2,3#   

  1. 1Biosecurity Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2 National Key Laboratory of Process Control and Intelligent Manufacturing Technology of Traditional Chinese Medicine Pharmaceuticals, Lianyungang 222000, China;
    3Jiangsu Kangyuan Pharmaceutical Co., Ltd, Lianyungang Jiangsu 222000, China
  • Received:2023-05-08 Online:2024-04-15 Published:2024-04-18

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摘要： 目的 观察佛芍颗粒对新斯的明诱导痉挛性腹痛大鼠模型的药效作用。方法 6周龄SD大鼠随机分为空白对照组,模型对照组,吗丁啉对照组（6.00 mg·kg-1）,四逆散对照组（1.50 g·kg-1）,佛芍颗粒高、中、低剂量组（24 g生药·kg-1、12 g生药·kg-1、6 g生药·kg-1）,每组10只,雌雄各半。采用腹腔注射新斯的明致大鼠痉挛性腹痛。观察大鼠疼痛潜伏期、疼痛持续时间和疼痛发生率,ELISA法检测血清P物质、胃泌素（gastrin,GT）和胃动素（motilin,MTL）含量,并观察大鼠胃组织和结肠组织的病理变化。结果 佛芍颗粒可以明显延长疼痛潜伏期（P<0.05,P<0.01）、缩短疼痛时长（P<0.01）并降低疼痛发生率,降低血清中P物质（P<0.01）、GT（P<0.01）和MTL含量（P<0.01）,减轻大鼠胃和结肠的黏膜增生、间质炎症及杯状细胞肿大和增多。结论 佛芍颗粒能够改善新斯的明诱导的痉挛性腹痛。

关键词: 佛芍颗粒, 新斯的明, 痉挛性腹痛, 药效, 大鼠

Abstract: Objective To study the effects of Foshao granules on spastic abdominal pain in rats. Methods Six-week-old SD rats were divided into the normal control group, model group, domperidone control group（6.00 mg·kg-1）, Sini powder control group（1.50 g·kg-1） and Foshao granule groups (24 g crude drug·kg-1, 12 g crude drug·kg-1 and 6 g crude drug·kg-1), with 10 mice in each. Spastic abdominal pain in rats was induced by intraperitoneal injection with neostigmine. The incubation, duration and incidence of pain as well as the pathological changes in the stomach and colon of the rats were observed, while serum contents of substance P, GT and MTL were detected by ELISA. Results Foshao granules could prolong pain incubation (P<0.05, P<0.01), shorten the pain duration (P<0.01), lower the incidence of pain, reduce the serum contents of substance P (P<0.01), GT (P<0.01), MTL (P<0.01), and inhibit mucosal hyperplasia, interstitial inflammation and the enlargement and increase of goblet cells in of the stomach and colon of rats. Conclusion Foshao granules can improve neostigmine-induced spastic abdominal pain.

Key words: Foshao granules, neostigmine, spastic abdominal pain, pesticide effect, rats

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