中国药物警戒 ›› 2022, Vol. 19 ›› Issue (12): 1315-1320.
DOI: 10.19803/j.1672-8629.20210458

• 基础与临床研究 • 上一篇    下一篇

彝族药姜味草饮片标准汤剂研究

王梦1,2,3, 支雅婧1, 郄兰霞4, 果佳慧1, 蔡于罗1, 牛丽颖1,2,3,*   

  1. 1河北中医学院,河北 石家庄 050091;
    2河北省中药配方颗粒技术创新中心,河北 石家庄 050091;
    3河北省中药材品质评价与标准化工程研究中心,河北 石家庄 050091;
    4石家庄市第五医院,河北 石家庄 050024
  • 收稿日期:2021-05-08 出版日期:2022-12-15 发布日期:2022-12-21
  • 通讯作者: *牛丽颖,女,硕士,教授·博导,中药分析及药效物质基础研究。E-mail:niuliyingyy@163.com
  • 作者简介:王梦,女,在读硕士,中药质量控制。
  • 基金资助:
    河北省自然科学基金资助项目（H2019423050）; 中央引导地方科技发展资金项目(206Z2501G); 河北省重点研发计划项目（20372502D）; 河北省中医药管理局项目（2022363）

Yi medicine Micromeria biflora (Buch.Ham.ex D.Don)Benth. standard decoction

WANG Meng1,2,3, ZHI Yajing1, QIE Lanxia4, GUO Jiahui1, CAI Yuluo1, NIU Liying1,2,3,*   

  1. 1University of Chinese Medicine, Shijiazhuang Hebei 050091, China;
    2Hebei TCM Formula Granule Innovation Center, Shijiazhuang Hebei 050091, China;
    3Hebei TCM Quality Evaluation & Standardization Engineering Research Center, Shijiazhuang Hebei 050091, China;
    4Shijiazhuang Fifth Hospital, Shijiazhuang Hebei 050024, China
  • Received:2021-05-08 Online:2022-12-15 Published:2022-12-21

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摘要： 目的 制备彝族药姜味草饮片标准汤剂,并对其质量进行研究。方法 制备15批不同产地的姜味草饮片标准汤剂,以迷迭香酸为定量检测指标计算转移率及出膏率,并建立高效液相色谱（HPLC）特征图谱的分析方法。结果 15批姜味草标准汤剂中迷迭香酸转移率范围为34.11%~104.72%,出膏率范围为11.90 %~15.10 %;采用《中药色谱指纹图谱相似度评价系统》（2012 版）软件对特征图谱进行分析,共标定23个共有特征峰,指认出其中3个色谱峰;通过与对照图谱比对,其相似度均>0.90。结论 本研究建立了姜味草饮片标准汤剂质量评价方法,且精密度、稳定性和重复性良好,可为姜味草的质量控制提供参考。

关键词: 彝族药, 姜味草, 迷迭香酸, 饮片, 标准汤剂, 特征图谱

Abstract: Objective To prepare Yi Medicine Micromeria biflora(Buch.Ham.ex D.Don)Benth. standard decoction and to study the quality standard. Methods Fifteen batches of Micromeria biflora(Buch.Ham.ex D.Don)Benth. standard decoction were prepared using the standard method. With rosmarinic acid as the quantitative detection index, the transfer rate and extraction rate were calculated and an HPLC characteristic chromatogram analysis method was established. Results According to the measurement of 15 batches of Micromeria biflora(Buch.Ham.ex D.Don)Benth. standard decoction, the transfer rate ranged from 34.11% to 104.72% and extraction rate from 11.9 % to 15.1 %. Similarity Evaluation System for Chromatographic Fingerprint of TCM (2012) was used to analyze and compare the characteristic chromatograms so that 23 common peaks were determined and 3 of them were identified. Moreover, the similarities were all over 0.90. Conclusion This determination method displays good precision, stability and repeatability, which can provide reference for quality control of Micromeria biflora(Buch.Ham.ex D.Don)Benth.

Key words: Yi medicine, Micromeria biflora(Buch.Ham.ex D.Don)Benth., rosmarinic acid, prepared drugs in pieces, standard decoction, characteristic chromatogram

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