The mechanism of “reducing toxicity and preserving effect” of curcumin - arecoline interaction based on weak bond chemistry

doi:10.19803/j.1672-8629.20230194

Abstract

Abstract: Objective The interaction between curcumin and arecoline and the formation of aggregates were observed on the basis of the commonly used matching drug pairs of traditional Chinese medicine, so as to explore the mechanism of “attenuating toxicity and preserving effect” during the matching process of traditional Chinese medicine and provide references for the safe use of arecoline compound preparations in clinical practice. Methods UV-vis, IR, ¹H-NMR, PXRD, ITC and conductance titration were used to analyze the formation process of the aggregation and characterize its structure. Acute toxicity test in mice and HepG2 cell model were used to observe the changes of toxicity and effect of the aggregation before and after formation. Results The results showed that arecoline and curcumin could form aggregation in 1:1 ratio by weak bond force in aqueous solution. This aggregate can significantly alleviate the stimulative effect of high-dose arecoline hydrobromide while maintaining the original anti-HepG2 proliferation activity of curcumin. Conclusion Based on the theory of TCM compatibility and the experience and practice of TCM clinical medication, this experiment provided a new idea for the study of “reducing toxicity and preserving effect” of TCM compatibility.

Key words: arecoline, curcumin, weak bond chemistry, reducing toxicity and preserving effect, the matching process of traditional Chinese medicine

CLC Number:

R994

LIN Xiaoyu, XIANG Jiawei, YAO Shuchang, TAN Senli, WEI Jichang, CHEN Ziyu, CHEN Siyu, LEI Haimin, WANG Penglong. The mechanism of “reducing toxicity and preserving effect” of curcumin - arecoline interaction based on weak bond chemistry[J]. Chinese Journal of Pharmacovigilance, 2023, 20(10): 1099-1107.

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