Determination of 87 types of pesticide residues in Polygoni Multiflori Radix by LC-MS/MS

doi:10.19803/j.1672-8629.20230085

Abstract

Abstract: Objective To establish a liquid chromatography-tandem mass spectrometry (LC-MS / MS) method for the determination of 87 types of pesticides and their metabolites in Polygonum Multiflori Radix in order to screen pesticides in 53 batches of samples of Polygonum Multiflori Radix, provide reference for safety study of Polygoni Multiflori Radix. Methods The samples were extracted with acetonitrile via high speed homogenate, concentrated and added with analytical protective agents before being determined with LC-MS/MS. The multi reaction monitoring mode (MRM) was used to collect data, and the blank matrix matching-protective agent standard was adopted for quantitative analysis to increase the accuracy and improve the peak shape. Results The calibration curves of all the targets showed good linearity with correlation coefficients of 0.99. The average recovery of the target compounds ranged from 70.4% to 107.4% at three spike levels, with relative standard deviations less than 11.40%, which met the requirements of trace determination. Carbendazim, acetamiprid and pyrazolidate were detected in 53 batches of samples of Polygonum Multiflori Radix, and the residues were all below 0.01 mg·kg^-1. Conclusion The detection indicators of this method involve the vast majority of banned pesticides detected by LC-MS / MS. This method is highly sensitive and accurate so that it can be applied to the screening and detection of pesticide residues in Polygonum Multiflori Radix, which can provide reference for the detection of pesticide residues in similar Chinese herbal medicines.

Key words: Polygoni Multiflori Radix, carbendazim, acetamiprid, pyraclostrobin, pesticide residues, LC-MS/MS, matrix effect, multi-response monitoring mode(MRM), safety

CLC Number:

R917
R994.11

LIU Yuanxi, GU Donglin, WANG Qi, YAO Lingwen, WANG Ying, JIN Hongyu, MA Shuangcheng. Determination of 87 types of pesticide residues in Polygoni Multiflori Radix by LC-MS/MS[J]. Chinese Journal of Pharmacovigilance, 2023, 20(6): 601-608.

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