Protective Effects of Wenyang Tongmai Granules against Oxidative Damage to HUVECs Cells

doi:10.19803/j.1672-8629.2020.12.02

Abstract

Abstract: Objective To explore the protective effect of Wenyang Tongmai Granule-containing serum against oxidative damage to human umbilical vein endothelial cells (HUVECs), and explore the possible mechanism.Methods Twenty-four rats were randomly divided into the blank serum group, low-dose drug-containing serum group (0.125 g/mL) and high-dose serum group (0.25 g/mL) with 8 rats in each. HUVECs were cultured in vitro to establish a normal group, a model group (300 μmol/L H₂O₂), a blank serum group (300 μmol/LH₂O₂+10% blank serum), and low-dose and high-dose Wenyang Tongmai groups (300 μmol/L H₂O₂+ 0.125 g/mL, 0.25 g/mL, 10 % drug-containing serum). MTT method was used to determine cell viability while the biochemical method was adopted to measure SOD activity and LDH release. MCP-1 and ICAM-1 mRNA expressions of endothelial cells were detected via RT-PCR.Results Compared with the model group, the cell viability and SOD activity of the Wenyang Tongmai groups increased (P<0.01), LDH release decreased (P<0.01), and ICAM-1 and MCP-1 mRNA expressions decreased significantly (P<0.01).Conclusion Wenyang Tongmai Granule-containing serum has a protective effect on HUVECs induced by H₂O₂. The mechanism may be related to its ability to reduce the expressions of inflammatory factors - ICAM-1 and MCP-1 mRNA. It can protect damaged endothelial cells and delay the progression of such vascular diseases as atherosclerosis.

Key words: Wenyang Tongmai granules, HUVECs, hydrogen peroxide, ICAM-1, MCP-1, atherosclerosis

CLC Number:

R285

ZHANG Li, HOU Lijing, SUN Fujun, LI Guihai, HUANG Dehong, SHENG Guoliang. Protective Effects of Wenyang Tongmai Granules against Oxidative Damage to HUVECs Cells[J]. Chinese Journal of Pharmacovigilance, 2020, 17(12): 856-860.

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