Abstract: Objective To establish the rapid detection method for imperatorin in Angelicae dahuricae Radix and the amounts of prim-O- glucosylcimifugin and 5-O-methylvisammioside in Saposhnikoviae Radix by near infrared spectroscopy (NIRS). Methods 100 batches of samples were collected from different regions, the content of label components were determined by high performance liquid chromatography (HPLC), and near infrared spectra of samples were also determined. Due to smaller differences between the spectral, it was needed to deal with spectrum by regression methods, pretreatment methods, bands as well as the numbers of principal components. The near infrared spectroscopy quantitative model were established for Angelicae dahuricae Radix and Saposhnikoviae Radix. Results In Angelicae dahuricae Radix, the calibration model of R² was 0.975 5, RMSEC and RMSEP were respectively 0.011 2 and 0.011 6, the principal component number was 11. In Saposhnikoviae Radix, the calibration model of R² was 0.977 3, RMSEC and RMSEP were respectively 0.054 5 and 0.051 4, the principal component number was 6. Conclusion The quantitative analysis models established in this research were more accurate and can be used to predict imperatorin in Angelicae dahuricae Radix and the amounts of prim-O-glucosylcimifugin and 5-O-methylvisammioside in Saposhnikoviae Radix, which have good application prospects.

Key words: near infrared spectroscopy, Angelicae dahuricae Radix, Saposhnikoviae Radix